FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20956583
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC877510.41872761413442255TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT116650.035.43688242
TCGTATG116800.035.2598843
AGTCACG123300.034.5211328
ACGTCTG126850.034.4911715
CGTCTGA129150.033.84208716
CGTATGC123250.033.39693544
TATCTCG127750.032.98541339
GTCACGC131250.032.41298329
ATCGGAA140050.031.6972942
TCGGAAG140200.031.6796573
CGCCAAT137200.030.95233733
CACGTCT142300.030.8728814
CACACGT145100.030.33906212
ACGCCAA140900.030.17479532
GTATGCC138300.029.9416345
ACACGTC148600.029.7153813
CAGTCAC144450.029.63853627
CACGCCA145200.029.28356631
TCTCGTA146450.028.55984341
GCCAATA149150.028.4217934
Produced by FastQC (version 0.11.5)