FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21788087
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC424310.19474403604134682TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG69850.029.46631415
TCGTATG67600.029.43133543
CTCGTAT67600.028.92828242
CGTCTGA72750.028.26079416
CGTATGC76900.025.96029744
ATCGGAA84000.024.96352
TCGGAAG84200.024.958163
CACGTCT88000.023.79787314
CACACGT88600.023.50977112
GTCACAC85350.023.47149529
CAGTCAC91200.022.18845627
AGTCACA92950.021.6734428
ACACGTC100200.021.1697213
GTATGCC97250.021.06028245
AGTGATC94150.020.83498636
TCTCGTA95300.020.80064241
CACAGTG96050.020.71177933
GATCGGA107050.019.780151
ACACAGT102150.019.69756532
CCAGTCA104650.019.53047626
Produced by FastQC (version 0.11.5)