FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19616462
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC361140.18410047642638108TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG64550.030.14299215
CGTCTGA65850.029.44543516
CTCGTAT59200.029.30688542
TCGTATG61650.028.10951243
ATCGGAA75150.026.6752992
CGTATGC65550.026.3685644
TCGGAAG80800.024.699453
CACACGT80100.024.5439412
CACGTCT81450.024.302914
AGTCACT79250.024.08597828
ACACGTC85050.023.53868313
ACTGACC83150.022.84623732
TCTCGTA80500.022.10893241
GATCGGA90550.022.0673071
CAGTCAC89200.021.70246327
AATCTCG84550.021.26084339
GTATGCC84750.020.84624545
CACTGAC94500.020.34205831
GTCACTG94850.020.31440529
GTCTGAA99950.019.55705617
Produced by FastQC (version 0.11.5)