FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11756355
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC170320.14487483578030777TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26200.028.16157515
TCGTATG24600.027.90048443
CTCGTAT25000.027.54151242
ACTTAGG26500.027.51855332
CGTCTGA27200.027.37432916
TCACTTA29700.024.48030730
TTAGGCA30150.024.17981334
AGTCACT29600.024.1066328
TCGGAAG32250.023.2318553
CTTAGGC32000.022.92683833
CACGTCT32700.022.70129414
CACACGT33600.022.42796912
GTCACTT32250.022.2653729
CGTATGC31550.021.89725744
ATCGGAA34200.021.7097782
CAGTCAC36550.020.32353227
TCTCGTA37450.018.98457741
ACACGTC41650.018.41720813
TAGGCAT40700.017.9632335
CACTTAG44750.016.29756731
Produced by FastQC (version 0.11.5)