FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285536
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC184812756.2503956736435TruSeq Adapter, Index 2 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG155030.4718560381015457TruSeq Adapter, Index 2 (100% over 51bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC139020.42312730708170604TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTCC121700.3704114031926602TruSeq Adapter, Index 2 (98% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94180.2866503365052156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGCC86980.26473610394164TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGGC83600.25444858921040586TruSeq Adapter, Index 2 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC81120.2469003535496187TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTCTGCC59710.18173594810709728TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGATGTATCTCGTATGCCG58760.17884448686606996Illumina PCR Primer Index 2 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGCATGCC54500.16587856593262104TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGCC40230.12244577444897879TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC36350.11063643801194083TruSeq Adapter, Index 2 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGCATCTCGTATGCC34210.10412304111110028TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCCTATGCC34040.10360562173112697TruSeq Adapter, Index 2 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGTATCTCGTATGCC33520.10202292715709095TruSeq Adapter, Index 2 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2079800.044.4458351
GTATGCC1940600.043.8260545
ATCGGAA2111400.043.808482
ATCGGGA5350.043.7403142
TCGGAAG2114250.043.728013
CGGAAGA2121400.043.5742654
GAGCACA2124550.043.4027029
ACACGTC2116100.043.4000913
CACACGT2120400.043.38952312
AGCACAC2123650.043.38521610
CGTCTGA2110950.043.3823616
CACGTCT2114400.043.37859714
GGAAGAG2130750.043.3753975
GCACACG2123150.043.3746411
ACGTCTG2112800.043.3741815
GAAGAGC2130450.043.368046
AAGAGCA2128550.043.3644757
GTCTGAA2113000.043.31471617
AGAGCAC2132200.043.2807438
TCTGAAC2115800.043.2095518
Produced by FastQC (version 0.11.5)