FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19983743
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC246540.12337028153334437TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42500.028.89915315
CGTCTGA45450.026.97391316
CTCGTAT42600.026.35846542
TCGTATG44550.026.06647343
TCGGAAG55000.022.5815323
CGTATGC52150.022.39768444
CACGTCT55800.022.25293714
ATCGGAA56850.021.8464162
CACACGT58800.021.46189112
CAGTCAC64050.019.57448227
GTCACAT63900.019.16211929
ACACGTC70500.018.15539213
AGTCACA68750.017.875828
TCTCGTA66200.017.503641
CGGAAGA76600.016.8606474
GATCGGA76050.016.3331971
TCACGAT77100.016.04700735
AGCACAC82300.015.880897510
GTATGCC75300.015.87046245
GAGCACA84100.015.3539879
Produced by FastQC (version 0.11.5)