FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17408980
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8550.020.6381472
TAGATCT4800.017.806233
TTCCCTA5050.016.9373998
GTGTAGA4700.016.75050530
GAGTGTA4600.016.62565428
AGTGTAG4600.016.62565429
GTAGATC4800.016.4009232
TCCCTAC5500.014.7158299
TCTCGGT5200.014.69739737
TTTCCCT9000.013.2645457
GTAGAAC3950.013.09255711
TGTAGGG6050.013.00787618
TGTAGAT6400.012.65261331
GTGTAGG6650.012.51101617
TCGTGTA6500.012.45390115
GTAGGGA6350.012.03885519
TCAGGTA4900.011.9518827
TCGGAAG12350.011.9005873
GATCTCG7750.011.893599535
CCCTACA6700.011.74358410
Produced by FastQC (version 0.11.5)