FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755687
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23950.09.5365962
GAACGCT24650.09.30535221
GTAGAAC34650.09.28087211
AATCGAA50900.08.7788965
GGAAAAT106850.08.4696221
GTACTCG45750.08.1468454
GCGTGAC29800.08.1235531
AGTACTC51400.08.0914053
AAAATCC75700.08.0320363
ACTTTCG28750.07.97827819
AATTCAC44700.07.97704555
TCCGTTA48500.07.78958619
TTCGATC51900.07.66871932
CGTTAAC48650.07.58072321
CCGTTAA50650.07.37005820
GCGATTT61500.07.3575541
GCCCTAT35800.07.285240710
GAAATCC66400.07.1826323
AAAAATC77500.07.1781242
AATCCGC39300.07.17810775
Produced by FastQC (version 0.11.5)