FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24564419
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23500.013.0867022
GTAGAAC39800.012.148313511
TCAGGTA50200.010.1354397
GCGTGAC33500.09.9275311
AATTGAC39900.09.7852135
GGAAAAT114050.09.5613781
GAATCGA36200.09.4849954
AAAATCC75600.09.2386343
AGTACTC58950.09.1640023
GTACTCG55950.09.1245294
GGTAGAA53400.09.09456310
CAGGTAG54050.09.0334838
TAGAACA53250.08.99587912
GAATTGA64550.08.9937114
AGGTAGA57150.08.73442659
AACGGAC20250.08.4700435
AATCCTT72150.08.4142045
GTGACTC40300.08.4131253
TACTCGA60200.08.3599845
AATCGAA48050.08.3133675
Produced by FastQC (version 0.11.5)