FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22123911
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44100.012.8503211
CAGGTAG52800.011.1227378
TCAGGTA55400.010.9725697
TAGAACA55900.010.25897512
AGGTAGA60100.09.9147569
GGTAGAA62700.09.610270510
AGAGATC105600.08.63048652
ATCAGGT99300.08.075626
GGAAAAT102950.07.9999271
AAAAATC85800.07.85458232
AAAATCC83700.07.8316973
AGATCAG106900.07.7341534
GAATTGA66000.07.5298814
AGTGAGA83250.07.32047118
AATTGAC40100.07.26006655
AACAGTG82300.07.159407615
CAGTGAG83050.07.1488317
GAAAAAT150950.07.11986061
GAACAGT86150.07.100505414
GTACTCG28450.06.90790464
Produced by FastQC (version 0.11.5)