FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23096546
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC33950.012.32055811
CGTGACT25200.012.114812
TCGGGGG57900.09.90059839
GTACTCG50000.09.8033534
TCAGGTA45550.09.7363977
GCGTGAC34900.09.4649731
AATCGAA43150.09.2050035
GAATCGA34750.08.9703464
GGGGGCC76250.08.89972344
TAGAACA48550.08.89402712
AGTACTC58550.08.8020673
GGTAGAA48100.08.74109910
CAGGTAG49150.08.6065518
TACTCGA56700.08.5177095
GGGGGGC145200.08.48097843
GTGACTC37700.08.4538483
AATTGAC36350.08.3194085
GAATTGA54250.08.2858724
TTCGGGG56300.08.14587238
GGGGGAA109450.08.1363421
Produced by FastQC (version 0.11.5)