FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383431
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC52350.012.41466211
CGTGACT22000.010.8954222
TCAGGTA64050.010.8609927
TAGAACA64600.010.26988412
CAGGTAG67000.010.1089168
GGTAGAA67900.09.90079910
GTACTCG47650.09.8127884
TACTCGA52300.09.0615035
GCGTGAC31250.08.9768711
AGTACTC56250.08.8400893
AGGTAGA77200.08.8252979
GGAAAAT120650.08.66299251
GAATCGA43900.08.54138854
AATCGAA50950.08.5043235
AGATCAG105300.08.4733214
GGTAGGC24750.08.4593687
AAAATCC86350.08.454673
GAATTGA60450.08.3328494
TAAATCG9500.08.0893153
AACAGTG88550.07.796985615
Produced by FastQC (version 0.11.5)