FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23750051
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25650.012.0789262
GTAGAAC32850.010.26849711
GCGTGAC34150.010.0709261
GTACTCG52750.09.7637424
TACTCGA54350.09.2597565
AAAATCC76350.08.9707673
AGTACTC59800.08.8830613
GGAAAAT113850.08.7047761
TCAGGTA46750.08.6199387
AATTGAC37150.08.2617935
GAATCGA40450.07.81832034
TAGAACA44400.07.80035612
CAGGTAG44900.07.76777848
AATCGAA49250.07.74416645
AAATCCT83950.07.72262764
GGCGAAT17750.07.5209061
GTGACTC42450.07.4549783
GGGAAAT58900.07.41410161
GAATTGA59450.07.3714654
AAATTGA78250.07.3036524
Produced by FastQC (version 0.11.5)