FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25262722
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45700.011.80982311
TCAGGTA58400.010.1004057
CGTGACT21450.010.0163262
TAGAACA58000.09.73239912
CAGGTAG60250.09.29933558
GGTAGAA61250.09.21362710
GCGTGAC29050.08.7235981
GAATCGA40500.08.6459244
AATCGAA51850.08.618515
GGAAAAT123550.08.5709081
CTCGAAT22800.08.5346811
AGGTAGA65900.08.4276839
AAAATCC77950.08.1777943
TAATCGA22950.07.8748454
GTACTCG52200.07.83326444
GAATTGA61200.07.7148874
AATTGAC39650.07.68433675
AGTACTC62400.07.5349783
AAATTGA86600.07.48684264
CATAAGC74100.07.44386057
Produced by FastQC (version 0.11.5)