FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23940005
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23950.014.63682
GCGTGAC36000.010.9363541
GTAGAAC41950.010.82842311
GTGACTC39000.09.9110783
TCAGGTA50050.09.7607857
GTACTCG57550.09.61691954
GGAAAAT114200.09.5698881
GAATCGA33850.09.2762264
AAAATCC76900.09.2004163
AGTACTC66400.08.8170063
TACTCGA64150.08.7248385
TAGAACA52700.08.66278712
GAATTGA57250.08.5230194
CAGGTAG56750.08.3658488
AAATCCT82200.08.1620624
GGTAGAA56200.08.1612210
AATCGAA44750.07.9178815
TGACTCT51650.07.8288494
GCTCTAG48900.07.81782411
CTCGAAT20350.07.78322271
Produced by FastQC (version 0.11.5)