FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n02_B1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n02_B1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23113122
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26250.014.9048862
GCGTGAC36450.011.73216151
GTGACTC42000.09.8492333
GTAGAAC35600.09.34895411
GTACTCG57500.09.0753934
GCTCTAG47250.09.04280311
AATTGAC40050.08.9601585
GGAAAAT97500.08.748841
TCAGGTA41600.08.7130717
GAATTGA55000.08.707524
AGTACTC62600.08.6302663
AAAATCC70800.08.0138473
GGTAGGC21700.07.9885827
CTCTATA54600.07.859953445
TGACTCT57150.07.82654764
AATCGAA44450.07.7685645
CACTCGT53200.07.73453535
AAAAATC60150.07.6701952
ACTTTCG18250.07.639701419
ACTCGTG53100.07.6212436
Produced by FastQC (version 0.11.5)