FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17408980
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA9650.036.1267316
ACGTCTG10000.034.862315
CACACGT12200.029.12136812
ACACGTC12700.028.33515713
CACGTCT12450.028.00088114
TCGGAAG17350.024.8957883
ATCGGAA17700.024.40352
AGCACAC15900.023.0519410
CAGTCAC9250.022.64832127
GATCGGA19800.022.1606981
CGGAAGA19900.021.7058124
CCAGTCA10700.020.84232926
GCACACG17550.020.4999911
CTCCAGT16050.018.6635924
AACTCCA19550.017.95965422
CTGAACT22850.015.35558819
GAACTCC23600.015.34570521
AGAGCAC24550.014.8384768
GAGACCG5550.013.79993331
TGAACTC26350.013.654420
Produced by FastQC (version 0.11.5)