FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755687
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC552680.23265165936897553TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG85000.032.521528
TCGTATG81300.032.40809643
CGTCTGA86300.032.21835716
CTCGTAT81500.032.1891842
ACGTCTG87600.032.09975415
CGTATGC85750.030.70042844
TCGGAAG96600.029.5085163
ATCGGAA97900.029.0938192
CACACGT101900.027.68320712
GTCACGA102250.027.05700329
CACGTCT105300.026.76815614
ACACGTC113300.025.1163413
CAGTCAC113800.024.66694327
TCTCGTA108750.024.18387241
GTATGCC113000.023.6355145
GATCGGA121650.023.6031821
GAGCACA130700.022.134539
CGGAAGA130750.022.111424
GTCTGAA127100.022.0530317
ACGATCA128200.021.8082632
Produced by FastQC (version 0.11.5)