FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24564419
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC324580.13213420598305214TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG78100.029.92693515
CGTCTGA79450.029.39010616
CTCGTAT59850.026.69140642
TCGTATG58200.026.28941243
TCGGAAG92000.026.1664563
ATCGGAA93000.025.7401052
GTCACAC91950.025.11749529
CACGTCT101850.023.34595714
CACACGT101950.023.23465312
CAGTCAC102250.022.7194127
CGTATGC65800.022.53506944
AGTCACA103750.022.32584828
ACACGTC110050.021.83126613
CACACTT112100.020.78334231
AATCTCG90600.020.63422839
GATCGGA118600.020.433751
TCACACT115350.020.13922730
ACTTGAA121100.019.5076834
CCAGTCA120250.019.31858426
CGGAAGA127850.018.987934
Produced by FastQC (version 0.11.5)