FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22123911
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC518620.23441605781183988TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG92650.031.95243815
TCGTATG78500.031.67106643
CTCGTAT80400.031.62123142
CGTCTGA96350.030.72541416
ATCGGAA103350.029.0833532
TCGGAAG104150.028.9244373
CGTATGC85150.028.7751944
CACACGT106800.027.88748212
CACGTCT109050.027.39466314
ACACGTC114200.026.21835713
GATCGGA116950.025.8598561
CAGTCAC117900.025.01251427
GTATGCC99950.024.78446445
TCTCGTA108350.024.0651941
AGTCACC126650.023.55106428
CGGAAGA134450.022.6574634
GTCTGAA134500.022.2946917
GAGCACA146650.021.0310869
CCAGTCA140650.020.99877526
GCACACG145950.020.57641611
Produced by FastQC (version 0.11.5)