FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23096546
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC915730.396479196499771TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG130500.037.9411715
CGTCTGA132250.037.43911416
AGTCACG131500.036.82000728
CTCGTAT127900.035.83382442
TCGTATG127300.035.5623243
ATCGGAA147350.034.1413842
CACGTCT146950.033.95417414
TCGGAAG148450.033.903413
GTCACGC144800.033.62460329
TATCTCG143100.033.10815439
CGTATGC136500.032.935144
CACACGT153400.032.65848512
CAGTCAC151450.032.26708627
CGCCAAT152850.031.61591133
ACACGTC159500.031.4813
ACGCCAA155250.031.02776332
CACGCCA159900.030.37884731
CCAGTCA164800.029.58486226
GATCGGA171900.029.519831
TCTCGTA157000.029.50556441
Produced by FastQC (version 0.11.5)