FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383431
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC488790.19256262086870762TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG75450.031.24700715
TCGTATG74250.030.4859343
CTCGTAT74150.030.22240642
CGTCTGA78400.029.84170516
CGTATGC81650.027.8885944
ATCGGAA91450.026.053272
GTCACAC89400.026.03369729
TCGGAAG92350.025.9214343
CACGTCT102300.023.63955114
CACACGT104200.022.88466512
TCTCGTA101150.022.28701241
ACACGTC109600.022.1060713
CAGTCAC108050.021.99844427
GTATGCC106500.021.80380445
ACACAGT108150.021.520132
AGTGATC105650.021.37825836
CACAGTG108300.021.36386533
AGTCACA109650.021.32847228
GATCGGA115250.020.8331031
CCAGTCA123650.018.98641626
Produced by FastQC (version 0.11.5)