FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23750051
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC422470.17788172328556262TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG72350.032.9273715
CGTCTGA76050.031.147916
CTCGTAT67500.030.09887942
TCGTATG67900.029.92320443
TCGGAAG85750.028.336483
ATCGGAA86000.028.1494642
AGTCACT85550.027.33800328
CGTATGC73350.027.20958144
CACACGT91200.026.36820812
CACGTCT92700.026.08725214
ACTGACC93350.024.93311532
ACACGTC100800.024.2587613
AATCTCG92700.023.85537139
CAGTCAC98450.023.82446727
GTCACTG104400.022.3372929
GATCGGA110200.022.094181
TCTCGTA97250.021.30625341
CACTGAC111850.020.80917531
GTCTGAA119150.020.25840617
CGGAAGA122300.020.1810074
Produced by FastQC (version 0.11.5)