FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25262722
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC294290.11649180163562738TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG55150.028.65208432
ACGTCTG57900.027.46902515
CGTCTGA59850.026.46128316
CTCGTAT53600.026.40364642
TCGTATG53750.026.03848643
CTTAGGC65900.024.18121333
TCACTTA67800.023.43916930
CGTATGC59500.023.33326144
TTAGGCA69300.023.01819634
TCGGAAG71750.022.4509093
ATCGGAA71800.022.3728282
AGTCACT75400.021.10645528
GTCACTT76050.020.74846829
CACACGT79650.020.39171812
CACGTCT79550.020.24767914
CAGTCAC87350.018.60550727
ACACGTC90100.018.1514813
TCTCGTA81600.017.8114741
TAGGCAT90750.017.6000635
CACTTAG91450.017.45127531
Produced by FastQC (version 0.11.5)