FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23940005
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC306660.12809521134185228TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG57000.029.71809615
TCGTATG52050.028.5731943
CTCGTAT53100.028.47344642
CGTCTGA59000.028.40567616
CGTATGC59050.024.8815644
TCGGAAG72650.023.5975273
ATCGGAA72500.023.522322
CACCGAT72200.023.25856431
ACCGATG74100.022.81408132
GATGTAT74300.022.34429435
TATCTCG73050.021.4339739
CACACGT80300.021.43112412
CACGTCT81250.021.2083214
CAGTCAC85200.020.0003927
TCTCGTA78000.019.78647841
GTATCTC83550.019.65535738
AGTCACC90200.018.8917228
ACACGTC95900.018.10921313
TGTATCT95700.017.51230837
GATCGGA99500.017.4141331
Produced by FastQC (version 0.11.5)