FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l06_n01_B1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l06_n01_B1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23113122
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC386180.16708257759380146TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG65650.031.38775315
CTCGTAT59700.030.601242
CGTCTGA67850.030.43633316
TCGTATG63900.030.10521743
CGTATGC71900.026.693344
ATCGGAA79200.026.333782
TCGGAAG79950.026.1986873
CACGTCT85400.024.5766814
CACACGT86450.024.12194412
CAGTCAC86500.023.86383627
GTCACAT90250.022.97203629
AGTCACA91800.022.43703528
TCACGAT93650.022.05130835
TCTCGTA86300.021.63717541
ACACGTC99550.021.2641413
ATCACGA103600.020.34935834
GATCGGA103450.020.3384461
CCAGTCA103250.020.01422326
CATCACG109750.019.29886233
AGCACAC110600.019.28223410
Produced by FastQC (version 0.11.5)