FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19706959
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN396870.20138571354413434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT12400.021.528832
TTCCCTA9050.017.9058918
TCCCTAC8800.016.8631029
TACACGA7600.016.2704213
GAGTGTA7100.015.84289428
GTAGATC7400.015.80742932
TCTCGGT7650.015.5743137
TAGATCT7350.015.30257633
AGTGTAG7550.014.60064129
GTGTAGA7800.014.42113230
TCGTGTA7900.014.22985115
GTGTAGG8900.013.13649817
TGTAGGG9100.013.09485518
TTTCCCT14750.012.8270897
GTAGAAC5900.012.1910311
TGTAGAT10000.011.69788531
TAGGGAA10600.011.66531720
CCCTACA12150.011.09980110
TAGCCGG1853.0473294E-410.9378136
GTAGGGA11150.010.68676419
Produced by FastQC (version 0.11.5)