FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20608359
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355690.17259501350883882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21100.013.3939362
TTCGATC40400.012.8154752
GAACGCT30350.012.743854521
GCGTGAC26100.012.1313621
ACTTTCG42500.010.74073319
GTAGAAC25750.010.30220311
GTACTCG48350.010.2288294
AGTACTC53250.09.9294733
GTGACTC31500.09.8991633
CCTTACC28200.09.8645681
GCCCTAT48250.09.69143810
ATAGGGG46700.09.68017434
CCTGGCT47800.09.6021638
TGCCCTA53350.09.1891959
TGGTAGG51250.09.12915427
TTAGGGT42950.09.0688967
ATTAGGG46900.08.9339916
GGAAAAT84850.08.9025951
GATAGGG51750.08.82271833
GTTCGAT72100.08.7830381
Produced by FastQC (version 0.11.5)