FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22222310
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN382260.1720163205355339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23800.013.204662
GTAGAAC32650.011.22339711
GCGTGAC32750.010.4969861
GTACTCG50200.010.43687254
TACTCGA56150.09.202255
AGTACTC59450.09.1220333
TCAGGTA44500.08.9047917
GGAAAAT103450.08.6794531
GTGACTC38600.08.6637663
AACGGAC16250.08.6088795
CTCGAAT17650.08.5854371
GAATTGA56000.08.5493664
GAATCGA34550.08.3665814
GGTAGAA45550.08.29162910
TAGAACA45450.08.16326812
CAGGTAG47700.08.1599388
AATTGAC37650.08.0905325
AATCGAA39800.07.71017365
AAAATCC71750.07.62124163
ATTGACC39550.07.3477746
Produced by FastQC (version 0.11.5)