FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22857114
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN394120.1724277176899936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45050.012.37609111
TCAGGTA53650.010.7854767
CAGGTAG59100.09.8219178
TAGAACA58250.09.76660512
GGTAGAA58550.09.75287110
GTACTCG43150.09.31603054
AGATCAG94900.08.6859714
GGAAAAT103500.08.6748031
TACTCGA48000.08.6491845
AGTACTC51350.08.3605093
AGAGATC107350.08.2979792
AGGTAGA69900.08.1395179
GAATTGA65000.07.99109364
GAATCGA41350.07.9738684
AATCGAA45000.07.87201175
AAAATCC75400.07.8514433
AATTGAC41750.07.5660665
AAAAATC74250.07.5515032
ATTGACC42600.07.456546
AGAACGG128800.07.43822963
Produced by FastQC (version 0.11.5)