FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25218812
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN434880.1724426987282351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44150.012.93373111
TCAGGTA54600.010.8871217
GGTAGAA55800.010.07221210
CGTGACT25250.09.9392552
CAGGTAG57400.09.8385238
TAGAACA58250.09.61200612
AATCGAA46200.08.8892025
GAATCGA42300.08.7029674
GGAAAAT105200.08.2125151
CGGAAAT21800.08.0922021
GTACTCG50900.08.0312084
AGATCAG98200.08.0036464
GCGTGAC32800.07.9985771
AGGTAGA74050.07.7743869
AGTACTC58000.07.63632063
GGTAGGC24550.07.61251937
ACACGCG26800.07.4816966
AGAACGG157150.07.47730353
GAATTGA62250.07.4739114
AGAGATC119250.07.45120332
Produced by FastQC (version 0.11.5)