FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24096259
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN414620.17206820361617128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25550.013.0979272
GTAGAAC32100.011.69562911
GCGTGAC34050.011.1600641
GTACTCG53750.09.9167264
ACTTTCG27300.09.63706319
AGTACTC57900.09.5235233
GGTAGAA43200.09.10673710
AATCGAA47700.09.0354655
TAGAACA42800.08.93124812
TCAGGTA43500.08.9029547
GTGACTC42000.08.878123
GGAAAAT91550.08.8450271
TCCGTTA55850.08.73691419
GCGATTT62700.08.3694111
CGTTAAC58200.08.34551521
ATAGGGG32700.08.32219234
GCTCTAG46950.08.28366811
CCGTTAA60600.08.20039620
CTCGAAT19750.08.1314071
AAAATCC73250.08.1139913
Produced by FastQC (version 0.11.5)