FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23033329
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN392930.1705919278971789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28600.013.2806282
GTAGAAC32050.011.99471211
GCGTGAC34600.011.5695661
GTACTCG57750.011.4965144
AGTACTC62350.010.7995393
TACTCGA64250.010.183525
GAATCGA35100.09.9079644
TCAGGTA43150.09.8093937
GGAAAAT99650.09.4640871
GTGACTC44650.09.3621523
AATCGAA43350.09.3161625
GGTAGAA45300.08.78407210
CAGGTAG46750.08.7589688
AAAATCC69850.08.6047973
GAATTGA59200.08.5447054
TAGAACA46500.08.46246112
AGCCTAG20100.08.28054325
GCTCTAG51150.08.26292811
TGACTCT54050.08.2307864
AGATCAG82050.07.87151434
Produced by FastQC (version 0.11.5)