FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24157587
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN407410.16864681062723688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27100.014.1823982
GCGTGAC36850.011.8449461
GTACTCG52650.010.4229474
AGTACTC58150.09.9475383
GTGACTC43600.09.5876153
TACTCGA57200.09.5456125
GCTCTAG50000.08.40802311
GTAGAAC33800.08.3806211
GGAAAAT96600.08.3346271
AATTGAC38450.08.1564835
GAATTGA58900.08.12836554
CTCGAAT19900.07.95533131
TGACTCT54800.07.953524
AATCGAA45700.07.84992275
AAAATCC67850.07.7594593
ATGTCTC122700.07.656075543
GGTAGGC22700.07.63732247
TATCCAG59450.07.62831545
GAATCGA35950.07.60097174
TCAGGTA42700.07.5402277
Produced by FastQC (version 0.11.5)