FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23399415
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN407280.1740556334421181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23250.014.0032532
GCGTGAC29650.012.3573071
GTAGAAC34100.010.6142311
TCACACG24800.09.61168915
GTGACTC36350.09.5113973
GTACTCG53350.09.3129154
AATTGAC36100.09.2505355
AATCGAA47250.09.121035
TCAGGTA43200.09.0685977
GGAAAAT100300.08.6363731
GAATCGA35350.08.4329954
AGCCTAG20550.08.31803725
GAATTGA56950.08.2483484
GCTCTAG44750.08.238873511
AGTACTC62000.08.2006473
TACTCGA58650.08.1175835
TAGAACA47500.08.04763412
ACACGCG24400.08.0324896
GGTAGAA46550.08.016829510
CTCGAAT20200.07.83754871
Produced by FastQC (version 0.11.5)