FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n02_E1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n02_E1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24994848
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN429270.17174339287840437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28700.013.7855292
GCGTGAC36400.011.9908831
GTAGAAC35050.011.16048611
GTACTCG63050.09.6351034
GTGACTC43150.09.4780673
GAATCGA35900.09.1843324
AAAATCC77500.09.1548233
AATTGAC42050.09.0681345
AGTACTC69800.08.9993563
GAATTGA63150.08.9761394
GGAAAAT117800.08.8117591
AATCGAA47200.08.7957545
GCTCTAG51400.08.61638311
TCAGGTA47300.08.6155447
TACTCGA71250.08.4868635
TAGAACA47750.08.09978912
CTCGAAT20300.08.0210581
TCACACG24150.08.00808515
AGTGTGA58000.07.94828114
AAATCCT87400.07.88093234
Produced by FastQC (version 0.11.5)