FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19706959
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13100.032.9660915
CGTCTGA13400.032.22804316
CACGTCT15800.028.0437214
CACACGT17200.025.36364412
ACACGTC17950.024.43393113
TCGGAAG23200.022.9760743
ATCGGAA23200.022.9754332
CAGTCAC15800.022.8101427
AGCACAC21000.021.20214810
CCAGTCA18450.019.90050526
GATCGGA27450.019.5882471
CGGAAGA27800.019.5783524
GCACACG25300.017.95414711
AACTCCA26650.016.21496622
CTCCAGT25100.016.150424
ACCGATC7350.014.70210634
GAGACCG8800.013.0542731
GTCCTAT5200.012.54474843
AGCGGTT9900.012.49284711
CCGAGAC9200.012.48669429
Produced by FastQC (version 0.11.5)