FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20608359
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC350520.17008632273923413TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG56800.032.43536415
CTCGTAT52950.032.04026442
AGTCACG57500.031.27924228
CGTCTGA58550.031.27380216
TCGTATG54600.031.23783943
TCGGAAG67900.028.0654283
CGTATGC60750.028.03844844
ATCGGAA69250.027.192822
GTCACGA69750.026.1084829
CACGTCT72850.025.65988314
CACACGT75050.024.93772912
CAGTCAC73650.024.75651627
ACACGTC83650.022.48151813
TCTCGTA80500.021.15749741
CGGAAGA90550.021.0451954
AGCACAC91700.020.92492710
GATCGGA91950.020.6066271
ACGATCA89700.020.57718832
GAGCACA94350.020.3617449
GTATGCC86600.020.05879445
Produced by FastQC (version 0.11.5)