FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22222310
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG53700.028.1083615
CGTCTGA55800.027.05052216
CTCGTAT42750.024.31631342
TCGTATG42050.024.18699543
TCGGAAG64450.023.842383
ATCGGAA69250.022.1241912
GTCACAC70150.021.37077529
CACGTCT72400.021.34545714
CGTATGC46650.021.2231844
CACACGT76550.020.0706712
CAGTCAC79700.019.12071227
ACACGTC83850.018.56484413
AGTCACA81500.018.42221828
AATCTCG68350.017.77481539
CACACTT87200.017.29545831
GATCGGA94300.016.5847511
CCAGTCA93600.016.20909326
TCACACT95500.016.1222830
TCTCGTA68200.015.736505541
ACACTTG96650.015.7203432
Produced by FastQC (version 0.11.5)