FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22857114
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT43850.023.14149942
TCGTATG43500.022.70794743
ACGTCTG52500.022.19495615
CGTCTGA56600.020.62693816
CGTATGC52500.018.90087344
TCGGAAG67600.017.8057123
ATCGGAA66850.017.5676542
CACGTCT67500.017.5293514
CACACGT72650.016.22477112
ACACGTC76250.015.69479413
CAGTCAC79450.014.70481327
TCTCGTA73500.014.14218141
GATCGGA84700.014.08083251
AGTCACC85450.014.04110128
GTATGCC75400.013.757297545
CGGAAGA94100.012.98259454
CCAGTCA93750.012.55796626
GTCTGAA98450.012.10996917
TCCAGTC98750.011.98974525
GAGCACA108450.011.6988939
Produced by FastQC (version 0.11.5)