FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25218812
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC580410.2301496200534744TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG88400.033.94532415
AGTCACG87400.033.66343328
CGTCTGA91250.032.7865116
CTCGTAT85300.032.0504842
TCGTATG86750.031.46407543
GTCACGC100600.029.40299829
ATCGGAA105250.029.0058652
TCGGAAG105750.028.8053573
TATCTCG98150.028.65272139
CGTATGC97200.028.08147644
CACGTCT108500.027.9678214
CAGTCAC111600.026.70656627
CACACGT114100.026.65415212
CGCCAAT110350.026.6159533
ACGCCAA111650.026.33064532
CACGCCA114450.025.8448431
ACACGTC118000.025.7925313
TCACGCC124300.024.1227830
TCTCGTA117050.023.81616841
GATCGGA129700.023.6468141
Produced by FastQC (version 0.11.5)