FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24096259
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC508040.21083770721421943TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG77750.033.35927615
CGTCTGA79350.032.51653316
TCGTATG77050.032.1233243
CTCGTAT76300.032.11348742
CGTATGC86000.028.72792644
TCGGAAG92650.028.5564143
ATCGGAA93650.028.0355112
CACGTCT95200.027.57538814
GTCACAC95200.026.67244329
CACACGT100200.026.10951412
CAGTCAC99800.025.60094327
ACACGTC107350.024.6849813
AGTCACA103100.024.5413428
CACAGTG102000.024.49204633
AGTGATC103900.023.75413336
CGGAAGA114700.023.3805454
GTATGCC109900.022.68523845
TCTCGTA109250.022.63282641
ACACAGT112750.022.3004132
CCAGTCA117500.021.80198126
Produced by FastQC (version 0.11.5)