FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23033329
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC441420.19164403026588125TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69950.030.88041142
TCGTATG72950.029.7665843
ACGTCTG85600.029.5116415
CGTCTGA88350.028.5166716
CGTATGC79500.027.14428544
AGTCACT94900.025.94921328
ATCGGAA102950.025.1091462
TCGGAAG103250.024.9930973
CACGTCT104900.024.36072514
ACTGACC101300.024.3531632
CAGTCAC104750.023.7669927
AATCTCG98900.023.74935739
CACACGT110400.023.18770212
TCTCGTA99850.022.19502441
ACACGTC117050.022.02426713
GTCACTG114850.021.774929
CACTGAC115900.021.461131
CCAGTCA121600.020.62175826
GACCAAT120950.020.36835
TGACCAA121550.020.23241834
Produced by FastQC (version 0.11.5)