FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24157587
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC415210.17187560992743192TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG72750.033.1781615
CGTCTGA75200.032.0673116
CTCGTAT68950.031.29630742
ACTTAGG76150.030.97765532
TCGTATG69850.030.54016543
TTAGGCA82300.028.6228234
CGTATGC74650.028.45585344
TCGGAAG85950.028.3742713
AGTCACT83750.028.32874328
TCACTTA83250.028.25553730
ATCGGAA87950.027.7286322
CACGTCT90650.026.99893814
GTCACTT89200.026.49695429
CTTAGGC91650.025.78318833
CACACGT95750.025.654912
CAGTCAC94150.025.39076227
ACACGTC106650.023.20166813
TCTCGTA99100.022.0685841
CGGAAGA117300.021.2512264
CCAGTCA113100.020.93757826
Produced by FastQC (version 0.11.5)