FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23399415
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC257190.10991300423536231TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG50000.029.33330215
CTCGTAT47300.028.01965742
CGTCTGA52500.027.63654916
TCGTATG48000.027.51810543
TCGGAAG63100.023.2821883
ATCGGAA62750.023.1964362
TATCTCG61900.022.38914339
GATGTAT65050.022.27265735
CGTATGC59300.022.23640844
ACCGATG65100.022.1638132
CACGTCT66250.022.10438714
CACCGAT66000.021.89648231
CACACGT70050.021.09792312
CAGTCAC71950.020.14829627
GTATCTC77350.018.38248338
AGTCACC81000.018.0083228
TCTCGTA75900.017.93471141
ACACGTC83150.017.88230513
GATCGGA87450.016.7249571
TGTATCT86000.016.58586937
Produced by FastQC (version 0.11.5)