FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l05_n01_E1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l05_n01_E1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24994848
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC264790.1059378316683502TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG50500.026.41475315
TCGTATG50500.024.59645743
CGTCTGA54350.024.54360616
CTCGTAT49600.024.08918442
CGTATGC57800.021.52900144
ATCGGAA67750.019.8576622
TCGGAAG68850.019.8021283
CACACGT70500.019.52741212
CACGTCT69350.019.49446114
GTCACAT76550.017.49650829
CAGTCAC77900.016.9910227
AGTCACA80350.016.66904328
ACACGTC87100.015.96079213
TCTCGTA79500.015.25458741
AGCACAC94450.015.07593110
GAGCACA97400.014.8507179
GATCGGA93000.014.7109051
CCAGTCA97850.013.68792126
GTATGCC94750.013.67948845
ATCACGA102150.013.45800834
Produced by FastQC (version 0.11.5)