FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18781869
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN659260.35100873081374384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10350.025.5421352
TCTCGGT4650.016.92246437
TTCCCTA7200.016.8787738
AGTGTAG4800.016.87253829
TCCCTAC6750.016.6613399
GTAGATC4800.016.39916232
GTGTAGA5000.016.19742230
GAGTGTA5000.015.74770428
TAGATCT5600.014.45749533
TTTCCCT12100.014.1445537
TACACGA6700.014.09778113
ATCGGAA13100.013.7984532
AGCGGTT7700.013.72526310
TCGGAAG13750.013.1401243
AACCGCT3650.012.9427331
CCCTACA8000.012.92949110
GTGTAGG7000.012.20799617
GATCGGA15900.011.656981
TCGTGTA6950.011.64876915
TAGGGAA7350.011.62663320
Produced by FastQC (version 0.11.5)