FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22798810
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN427630.187566807214938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23400.011.9720362
GTAGAAC29450.011.37666511
GCGTGAC29800.010.6936111
GTACTCG54550.010.3416194
TACTCGA57050.09.6433445
AGTACTC63250.09.4967733
TCAGGTA40050.09.1644447
GGAAAAT101300.08.7680841
GAATTGA60400.08.293914
TAGAACA41950.08.09525712
GTGACTC36500.07.9808983
TATAAGG25900.07.9220055
AAAATCC81400.07.9063383
AATTGAC43500.07.8785775
GGTAGAA46350.07.85916510
CAGGTAG48050.07.6343888
AAATCCT81100.07.56817774
AATCCGC32000.07.5392355
CTATAAG26050.07.53624064
AGAACGG106050.07.36750363
Produced by FastQC (version 0.11.5)