FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25462775
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC35150.09.21190711
CGTGACT25200.08.8767462
GGAAAAT115300.08.3320111
GTACTCG47800.08.1678264
AATCGAA49700.08.0758945
TACTCGA48250.07.9914545
AAAATCC83000.07.94485243
GCGTGAC32750.07.93737171
CCCGAAT18000.07.9114891
AATTCAC44150.07.55889035
TCAGGTA47850.07.48200857
AATTGAC44500.07.34742265
AGTACTC54700.07.22490023
GGGGAAT55950.07.1509441
GAATTGA74050.07.10099364
CAGGTAG47300.07.0892378
ACAATCG20700.06.98217543
AATCGTA12650.06.95186145
AAATCGA52650.06.94392354
AAAAATC80900.06.9266352
Produced by FastQC (version 0.11.5)