FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23501528
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN425600.1810946079761282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40100.011.43929511
TCAGGTA50950.09.7223157
GGTAGAA50650.09.23411910
ACTTTCG31950.09.15045119
CAGGTAG51550.09.1241948
TAGAACA52900.08.8002112
GCCCTAT36100.08.65803210
AGTCTCG24350.08.61179355
ATAGGGG33950.08.61124934
CGTGACT21750.08.51831152
GGAGAAT90900.07.98168561
GGAAAAT91700.07.9120531
AGGTAGA60250.07.8772769
GGGGAAT54700.07.85090641
CCTTACC24300.07.81315661
GTACTCG33800.07.67812544
TAAGTCT27600.07.52788263
TAGGATA40150.07.508293630
TGCCCTA44650.07.5061199
TTAAGTC29850.07.49358752
Produced by FastQC (version 0.11.5)